Hello again, and thanks for answer me.
Well, I chance some products of my transfer buffer, and the way that I make the "sandwich step", and now the thinks are ok again!!!
I think the problem was in the transfer step! Thank you Admin.
Admin, to transfer 30v overnight, what mA should I need to use?
Anastasia_S, my transfer stills 60 V (55-60v), 200 mA (for 1 gel) for 2 hours on ice, and I think it's enough time to transfer. I didn't have problem anymore.
Maybe for protein whit high molecular weight, you'll need more time. I can transfer in 2 hours, proteins with 70-72 kda for example.
See you soon!!!