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Western Blot Forum Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


pvdf membrane

pvdf membrane - Western Blot Forum

pvdf membrane - Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


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Old 04-29-2009, 08:49 AM
Pipette Filler
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Default pvdf membrane



PVDF membrane is incubated with antibodies, washed and dried. Then, I need to take it to a distant lab, where I'll be able to detect the blots using Storm Amersham. I would activate it there and prepare it for detection. Has somebody done this, without having trouble detecting the signal? Is it better to carry it dried, or in the buffer solution? Do I need to take it in a cooler or not?
ps: The other lab is an hour ride.



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Old 07-30-2009, 05:11 PM
Pipette Filler
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Default Re: pvdf membrane

I've transported blots in buffer solution for development somewhere else with no problem. I haven't done it with a dry membrane or used Storm Amersham though - so I can't comment on that.
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