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| Western Blot Forum Discuss western blotting and immunoblot in the western blot forum. |
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04-27-2009, 01:35 PM
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 weird bands visible on blot after ECL Hello, I sometimes have bands appearing after ECL, visible on the blot, but w/o need to use camera just visible by eye...someone could tell me why? thanks vini  |
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05-29-2009, 12:55 AM
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| Re: weird bands visible on blot after ECL Hello, the glowing of extra bands has happened to me also. You should check your secondary antibody concentration / dilution and bring it a bit down and/or increase your blocking conditions (add up to 5% blotto milk) during your secondary antibody incubation.  welcome to the forums by the wayCheers |
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05-29-2009, 12:46 PM
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| Re: weird bands visible on blot after ECL They appear on the exact place of the cemiluminescent bands? Lol, I'm probably wrong, but I always thought, that HRP+substrate kind of burn it out Although it does disappear after stripping |
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| bands , blot , ecl , visible , weird |
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