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Western Blot Forum Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


best lysis buffer to use

best lysis buffer to use - Western Blot Forum

best lysis buffer to use - Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


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Old 04-23-2009, 02:51 PM
Pipette Filler
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Default best lysis buffer to use



Hi,
I have been doing western blots and I always have multiple bands. I changed some conditions but what I did not change the lysis buffer.
Could you please let me know of the recipe/quick protocol for a good lysis buffer that normally works.
Thank you so much, that would help a lot
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