| | Re: Why the band at wrong molecular weight?
Thank you for your kind suggestion.
The protein here is actually HIF1-alpha, which is extremely vulnerable to degradation. In literature it appears a band at around 120 kDa. But you know, most people won't show the entire gel, so I have no idea if they also have a 70kDa band. It is possible that the 70kDa band is the degraded form of HIF1-alpha. Is there any way to check it, rather than blindly taking more care of prevention of protein degradation? Thanks!