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why white bands instead colored one in WB?

why white bands instead colored one in WB? - Western Blot Forum

why white bands instead colored one in WB? - Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


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Old 03-26-2009, 06:00 AM
Pipette Filler
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Default why white bands instead colored one in WB?



I am getting white band where the proteins I am interested in are located, how I can solve this probles? is it related with too much protein in the membrane?a lot of primary antibody or secondary one? I am using AP GAM secondary antibody in dilution 1:6000 and monoclonal antibody in 1:200.
Could you help me please?
Thanks a lot
westwoman
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Old 03-31-2009, 09:51 AM
Pipette Filler
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Default Re: why white bands instead colored one in WB?

Hi, I once also encountered this problem, and I solved it by reducing the concentration of secondary antibody. Maybe you can have a try. Good luck!
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