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Western Blot Forum Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


immunoglobulin depletion

immunoglobulin depletion - Western Blot Forum

immunoglobulin depletion - Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


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Old 02-02-2009, 02:37 PM
Pipette Filler
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Question immunoglobulin depletion



Hello,

I am currently performing western blots on tissue extracts, the protein I am looking for has different MW's depending on monomer, dimer or proprotein structure, however 2 of these forms are at the same height as the light and heavy chain of Ig's. My secondary antibody is raised in the same species as my protein extracts, thereby recognizing very well the Ig's. Somebody already tried depletion of immunoglobulins in order to get rid of these unspecific bands, still keeping the western blot +/- quantitative (relative expression)?
I wonder whether I could use protein L agarose or something alike?
Any other suggestions?

greetings Mel D
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