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Western Blot Membrane Stripping Reprobing Disadvantages

Western Blot Membrane Stripping Reprobing Disadvantages - Western Blot Forum

Western Blot Membrane Stripping Reprobing Disadvantages - Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


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Old 01-28-2009, 04:47 AM
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Default Western Blot Membrane Stripping Reprobing Disadvantages



Does anyone know any disadvantages of Western Blot membrane Stripping & Reprobing please?
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Old 03-31-2011, 07:40 PM
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Default Re: Western Blot Membrane Stripping Reprobing Disadvantages

sometimes results are not reproducible after stripping.If you need to compare in between samples,you have to be careful.So next time if you repeat your expt,start blotting with the Abs you use later. For some 2D gels stripping did not work at all in my case.Frustrating,specially if it takes lots of work and time, rare experience though.One should be careful to indicate protein conc after stripping.Hope this will help you.
MB
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