Hi, ive a blot that I'll work on first with Primary Ab that is monoclonal raised from goat. Then as secondary Ab, I'll use "rabbit anti-goat Ab".
Next I am planning to work on the same blot, this time with Primary Ab that is monoclonal raised from rabbit. Then secondary Ab, I'll use "Goat anti-rabbit Ab".
Do you think I should strip blot for the latter before proceeding? What worries me is that the secondary "Goat anti-rabbit" will interact with residual of secondary "Rabbit anti-goat".
Also do you think the protein interaction of secondary to primary Ab will break during the re-probing steps or that it's better to just STRIP?