Western Blot Woes - Very Clean Western

kiki06 · #1 10-03-2006, 05:44 PM

Hi everyone,
my western blot is clean, too clean! i dont see anything...i think i know the problem but does any one have anymore ideas?

thanks in advance

kiki

anoopbal · #2 02-24-2007, 01:46 PM

Did you get the molecular weight markers?

Try staining the membrane and see if you have anyproteins.

rimapant · #3 12-01-2007, 04:06 PM

Is there any reversible method of staining to see the protein in the Blot

George · #4 07-22-2008, 02:30 PM

I had this same problem, too. It turned out to be our ECL problem.

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Western Blot Woes - Very Clean Western

Western Blot Woes - Very Clean Western - Western Blot Forum

Western Blot Woes - Very Clean Western - Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.