Western Blot Woes - Very Clean Western
 

Hi everyone,
my western blot is clean, too clean! i dont see anything...i think i know the problem but does any one have anymore ideas?

thanks in advance

kiki :)

Re: Western Blot Woes - Very Clean Western
 

Did you get the molecular weight markers?

Try staining the membrane and see if you have anyproteins.

Re: Western Blot Woes - Very Clean Western
 

Is there any reversible method of staining to see the protein in the Blot

Re: Western Blot Woes - Very Clean Western
 

I had this same problem, too. It turned out to be our ECL problem.