Good news guys.
I bought some ponceau s and stained my membrane after transfer at 15 V for 45 minutes.
BANDS WERE THERE YES!!!!!
Thanks for all your help guys.
Next step is to get this WB working .... haha,. Best of luck to all your experiments.
FOR ANYONE WHO HAS THE SAME PROBLEM:
- as people have told me in this thread, because protein is still in the gel doesn't mean that NO protein transferred.
- check membrane with some reversible stain if you're not sure
- once you're sure, just keep the same run time/volt etc.