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09-08-2008, 11:43 AM
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 Cytochrome C Detection Western Blotting Hey everyone I have to detect Cytochrome c release before and after apoptosis as a marker of programmed cell death PCD.  Does anyone have or know of a specific protocol for mitochondria isolation and is RIPA isolation sufficient?  cheers   |
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09-09-2008, 08:57 PM
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 Re: Cytochrome C Detection Western Blotting I had experienced a hard time to detect cytochrome c on western blot using the tris-glycine transfer buffer. Would you please keep me posted on how your experiment goes? Concerning mitochondria isolation, I know there're several mitochondria isolation and/or purification kits on the market for that purpose. I haven't used them myself, but I think QIAGEN's should be good. |
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| blotting , cytochrome , detection , western |
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