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Cytochrome C Detection Western Blotting

Cytochrome C Detection Western Blotting - Western Blot Forum

Cytochrome C Detection Western Blotting - Discuss western blotting and immunoblot in the western blot forum.



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Old 09-08-2008, 11:43 AM
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Cool Cytochrome C Detection Western Blotting

Hey everyone

I have to detect Cytochrome c release before and after apoptosis as a marker of programmed cell death PCD.
Does anyone have or know of a specific protocol for mitochondria isolation and is RIPA isolation sufficient?

cheers
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Old 09-09-2008, 08:57 PM
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Default Re: Cytochrome C Detection Western Blotting

I had experienced a hard time to detect cytochrome c on western blot using the tris-glycine transfer buffer. Would you please keep me posted on how your experiment goes? Concerning mitochondria isolation, I know there're several mitochondria isolation and/or purification kits on the market for that purpose. I haven't used them myself, but I think QIAGEN's should be good.
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