Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protein Science > Western Blot Forum
Register Search Today's Posts Mark Forums Read

Western Blot Forum Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


Mouse Brain Western Blot Detection

Mouse Brain Western Blot Detection - Western Blot Forum

Mouse Brain Western Blot Detection - Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 09-08-2008, 10:41 AM
Denise's Avatar
Pipette Filler
Points: 2,395, Level: 31 Points: 2,395, Level: 31 Points: 2,395, Level: 31
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jan 2006
Posts: 21
Thanks: 0
Thanked 0 Times in 0 Posts
Unhappy Mouse Brain Western Blot Detection



hey dudes,
im trying to detect a nuclear transcription protein in mouse brains (adult).

I can only use SDS-PAGE and western blots however im getting issues detection the damn proteins using western!
There are a lot of unspecified bands on the gel instead of my band of interests.

Im using a simple lysis buffer composed of 1% NP-40, Tris, MgCl2, eg.

Please let me know what i may be doing wrong

thanks
Reply With Quote
  #2  
Old 09-17-2008, 02:52 AM
danfive's Avatar
Nobel Laureate
Points: 8,612, Level: 64 Points: 8,612, Level: 64 Points: 8,612, Level: 64
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jul 2007
Location: Houston TX
Posts: 969
Thanks: 12
Thanked 184 Times in 154 Posts
Default Re: Mouse Brain Western Blot Detection

Are you getting unspecific bands on the Western or the gel?
Your buffer is very weak in my opinion--how about SDS or sarkosyl?
Reply With Quote
Reply

Tags
blot , brain , detection , mouse , western


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Politics And Cannibalism? Introducing The Dourties, Chelsea, Bill, Hillary, Barrack Obama, George Bush, Jr., And All Of Capital Hill! jon_johnfrancisayres@yahoo.com Microbiology Forum 0 10-06-2007 05:59 AM
Western blot detection of membrane proteins Samuel C McNeely Cell Biology and Cell Culture 1 03-11-2007 03:02 PM
Biophysics & Schumann Resonance Consc Physics Forum 16 12-27-2004 10:45 AM
long mouse DNA template PCR, western blot with mouse tail Ripley Protocols and Methods Forum 4 10-17-2003 11:23 AM


All times are GMT. The time now is 01:09 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.14230 seconds with 16 queries