|Register||Search||Today's Posts||Mark Forums Read|
|Western Blot Forum Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.|
| ||LinkBack||Thread Tools||Display Modes|
Antibody Reuse for Western Blots?
We are experiencing some difficult problems with western blots.
After analysis we think that the problem might be due to the reuse of our antibodies.
Has anyone already had this sort of issue? Is antibody reutilization recommended for western blotting? How do you reuse / store them?
thanks in advance
Re: Antibody Reuse for Western Blots?
it really depends on the antibody you are using. We often reuse our antibodies, however some are only good for one western blot! It really depends on the quality and stability of the antibodies and how they are stored.
What problems exactly do you have with the reuse - no bands / light bands?
|antibody , blots , reuse , western|
|Thread||Thread Starter||Forum||Replies||Last Post|
|Custom antibody does not work as expected||jakekei||Antibody Forum||0||12-14-2009 09:53 PM|
|SUMO antibody problem (Nikola Wenta)||Suresh Kumar||Protocols and Methods Forum||0||05-19-2009 07:52 PM|
|Production of Monoclonal Antibodies - Technique in Mouse||Cellbiogal||Antibody Forum||2||01-08-2009 09:52 PM|
|Antibody Microarray Reuse?||Denise||Microarrays Forum||0||07-29-2008 03:10 AM|
|[Zbrafish] Fw: ask for antibody||houjia||Zebrafish Forum||0||09-12-2007 04:22 AM|