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Please clarify me regarding loading standard.

Please clarify me regarding loading standard. - Western Blot Forum

Please clarify me regarding loading standard. - Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


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Old 07-23-2008, 04:03 PM
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Default Please clarify me regarding loading standard.



I am in a need to do western blot for HSP 25 in serum since there is no kit available for HSP 25 in market to follow HSP through ELISA protocol.

What is the loading standand i have to look into (like tubulin, actin...) when i am doing western blot for HSP 25 in Serum.
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Old 08-02-2008, 05:05 AM
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Default Re: Please clarify me regarding loading standard.

Hi Bio,
i would look at several HSP 25 papers or similar genes and see what they use.

Usual standards are as you mention tubulin or actin, however i would actually try these in your conditions and see if they vary at all with treatment.
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Old 08-05-2008, 11:29 PM
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Default Re: Please clarify me regarding loading standard.

Thanks, the problem is Actin and others are in cell (cytosolic).
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