Sample Buffer

[abelaksono]

Anyone can explains regarding to the kind of sample buffer? Are there any expire date for using it?

I used two kind sample buffer, NuPAGE-Invitrogen and Sample Buffer Solution (2MF+)(x4)-Wako. When I used the last thing, the Western Blot results showed the curved bands results

Do anyone have suggest for me?

Regards,

Anom

[admin]

Hello Abel,

the best buffer i ever used was home made laemmli buffer. Very smelly but the best. read more about it here:
http://www.molecularstation.com/wiki/Laemmli_buffer

even includes a buffer recipe.

I guess your Wako sample buffer had weird salt or pH causing the migration curving. Also make sure to load sample buffer into all (even empty wells) to prevent curving.

[abelaksono]

Thanks anyway for your suggest. I consider at your opinion about pH and salinity (because this wako is about 4 years old in my lab).

Regards,
Abel