Anyone treating Cell lysates with RNAse/DNAse

[danfive]

I freeze and reuse leftover cell lysates for Western Blots and they become more viscous over time. I know TCA/Acetone precipitation can help stabilize the proteins etc but seems overkill.
Does anyone treat lysates (for use on Westerns) with RNAse and DNase to reduce viscosity? Got any recommendations on how many units of enzyme, incubation time, etc.? I usually use either Tris-Cl-SDS or CHAPS lysis buffer.

Thanks

[admin]

Hello Dan,
i would try pipetting the samples up and down quickly a few times with the yellow pipette tips for the P20-P200.

I had this problem with pcr samples however pipetting quickly sheared the DNA. I am pretty sure that most of the viscosity is caused by the DNA which can be sheared physically with pipetting. Someone please butt in if I they have any other ideas or comments.