Here's one another beginner question. I am about to do my first WB tomorrow and am wondering about loading controls.
You run ur protein samples on a gel with a ladder... and then do western using primary antibodies against ur protein of interest
and then secondary antibody. Where does the loading control come in picture?
I bet its a silly question but I was thinking you load duplicate wells and
slice ur gel after it has run, and probe with antibodies against target and say actin...simultaneously.
Thanks for any suggestions.