Multiple Antibodies at once?

[rlund]

I have a quick question, Im running an Assay which requires multiple antibodies. Every westerblot protocol I look up does not comment if I can mix more then 1 antibody and use on my westerblot at the same time. Does anyone know if I would get some cross linkage if i mix 2 together?

Thank you

[danfive]

Search kinetworks multiblotting for examples. But in short you can incubate all primary antibodies simultaneously and then secondary detection antibodies.