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| Western Blot Forum Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures. |
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| Hi all: I recently moved into a new lab and the protocol here is to run/transfer 1 gel/membrane, probe a 1' and 2' for one protein, then rinse in TBS-T for 20 mins, then probe a different protein, such as a loading control on the same membrane. Is this right? Thoughts on if this will work? Thanks J |
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