Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protein Science > Western Blot Forum
Register Search Today's Posts Mark Forums Read

Western Blot Forum Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


1 gel, 2 probes

1 gel, 2 probes - Western Blot Forum

1 gel, 2 probes - Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 02-27-2008, 08:04 PM
Pipette Filler
Points: 691, Level: 13 Points: 691, Level: 13 Points: 691, Level: 13
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Feb 2008
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Default 1 gel, 2 probes



Hi all:

I recently moved into a new lab and the protocol here is to run/transfer 1 gel/membrane, probe a 1' and 2' for one protein, then rinse in TBS-T for 20 mins, then probe a different protein, such as a loading control on the same membrane. Is this right? Thoughts on if this will work? Thanks

J
Reply With Quote
Reply

Tags
gel , probes


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Development of Fluorescent Probes otava Molecular Biology Techniques 1 09-23-2009 10:22 AM
[Zbrafish] Need insitu probes Burdine, Rebecca D Zebrafish Forum 0 02-11-2009 05:38 PM
commercially available in situ probes Arne K Christensen Protocols and Methods Forum 0 10-10-2008 06:47 PM
Problems doing Northern Blot with small probes (oligo, riboprobe,PCR and random primed) Sharon Protocols and Methods Forum 1 02-12-2008 08:44 AM
Design of in-situ probes for closely-related genes borsani.unibs@gmail.com Zebrafish Forum 0 08-31-2006 10:09 AM


All times are GMT. The time now is 06:42 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.10950 seconds with 16 queries