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Recommended Western Blot Membrane Supplier

Western Blot Forum

Discuss western blotting and immunoblot in the western blot forum.



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  #1 (permalink)  
Old 02-19-2008, 05:13 PM
Pipette Filler
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Question Recommended Western Blot Membrane Supplier

Hello,
i will be doing western blots for phospho-proteins. does anyone have any ideas on western blot membrane suppliers that have sensitive blot results?

thnx
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  #2 (permalink)  
Old 02-21-2008, 07:29 PM
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Default Re: Recommended Western Blot Membrane Supplier

I just switched from BIO-RAD to GE Healthcare. Actually works better.
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Old 02-22-2008, 06:30 PM
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Default Re: Recommended Western Blot Membrane Supplier

If you use nitrocellulose membrane its possible that the proteins go through the membrane. Its better to use PVDF-membranes because the proteins can't go through it.
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