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11-04-2008, 12:58 PM
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 Loading control for membrane fractions I am trying to quantify the membrane expression of a heterologously expressed protein in CHO cells. All cell surface proteins are biotinylated, pulled down and then analyzed by Western Blot. Does anyone have an idea what I could use as a loading control? Standard controls like actin, tubulin or GAPDH won't work, because the proteins need to be at least partially on the extracellular side to get biotinylated.  |
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10-17-2009, 10:29 PM
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 Re: Loading control for membrane fractions I routinely use Na/K ATPase antibody (sc21712) as a loading control for membrane fractions. I think cadherin or integrins could be other alternatives. |
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| control , fractions , loading , membrane |
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