Hiya, cool question and welcome to the forums.
Are you loading the same amount of protein in gels 1 and 2?
It seems that even though you may be loading the same amount of protein, the band intensity and the presence of extra bands changes from western 1 to western 2 (less bands).
You may be loosing some when lysing or loading, or the antibody concentrations (primary or secondary) may be different this time.
In general though, it is hard to say. I thought that the prepared gels from companies were pretty consistent however this may not always be the case.
Something to consider also is salt concentration. You should try keeping the salt concentration the same, and also make sure you load sds loading buffer into all the lanes even the empty ones and throw some into the marker lane just in case after loading the ladder.
Sometimes, the bands run in a smilie or weird curved lines because of missing sds loading buffer (or empty lanes).
Anybody else have a different opinion?