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| Western Blot Forum Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures. |
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#1
01-12-2008, 03:19 AM
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 Question on cold pack why is a cold pack added to the transfer assembly ? If at the end of the transfer process you notice that the transfer assembly and buffer are quite warm should we be concerned?  |
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#2
01-12-2008, 04:48 AM
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 Re: Question on cold pack Hello, we even transfer the western blots sometimes at room temperature, and they are ok. The ice pack will melt, meaning that it did its job in cooling the buffer/gel just enough to transfer it. However, the best thing to do is put it in the cold room at 4C, even there the ice pack/cold pack will melt eventually because its above zero. Are your bands/gel ok?  |
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