Internal controls for WB

[JINCAO]

I am new to this forum, and I found it is very good site to get advice on specific issues.
I'm working on a project to examine gene expressions in mouse tissues (liver, muscles etc) by Western Blots. I'm wondering which internal controls I should use? actin? tubulin? GAPDH? or something else?

[admin]

Hello there,
welcome to the forums.

I think it depends on which tissue you are using and how you are treating the cells. It pays to check similar publications and other publications to see what others are using, and if your treatment is affecting the controls in any way. If it is, then you may have to use a different control.

Are you treating the cells with anything?

cheers

[JINCAO]

No treatment. I'll compare gene expression cross different species of mouse.

[jrscientist]

I'm glad you started this post, as I have a similar question I'd like to throw out (sorry, no answer).

Please see my thread here:
[Only registered users see links. ]

[gandalfthegrey]

Hello there,

I have has similar issues with western blots.

The best thing to do is try two internal controls such as alpha tubulin and beta actin.

Then you can use one as a loading control and the other as an internal control


Always check to see if any of the internal controls are affecting in different tissues or treatments.