Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protein Science > Western Blot Forum
Register Search Today's Posts Mark Forums Read

Western Blot Forum Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


Internal controls for WB

Internal controls for WB - Western Blot Forum

Internal controls for WB - Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 12-18-2007, 04:50 PM
Pipette Filler
Points: 816, Level: 16 Points: 816, Level: 16 Points: 816, Level: 16
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Dec 2007
Posts: 3
Thanks: 0
Thanked 0 Times in 0 Posts
Question Internal controls for WB



I am new to this forum, and I found it is very good site to get advice on specific issues.
I'm working on a project to examine gene expressions in mouse tissues (liver, muscles etc) by Western Blots. I'm wondering which internal controls I should use? actin? tubulin? GAPDH? or something else?
Reply With Quote
  #2  
Old 12-18-2007, 05:12 PM
admin's Avatar
Administrator
 
Join Date: Nov 2005
Posts: 1,418
Thanks: 883
Thanked 68 Times in 58 Posts
Default Re: Internal controls for WB

Hello there,
welcome to the forums.

I think it depends on which tissue you are using and how you are treating the cells. It pays to check similar publications and other publications to see what others are using, and if your treatment is affecting the controls in any way. If it is, then you may have to use a different control.

Are you treating the cells with anything?

cheers
Reply With Quote
  #3  
Old 12-18-2007, 06:33 PM
Pipette Filler
Points: 816, Level: 16 Points: 816, Level: 16 Points: 816, Level: 16
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Dec 2007
Posts: 3
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: Internal controls for WB

No treatment. I'll compare gene expression cross different species of mouse.
Reply With Quote
  #4  
Old 12-20-2007, 06:00 PM
Pipette Filler
Points: 829, Level: 16 Points: 829, Level: 16 Points: 829, Level: 16
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Dec 2007
Posts: 2
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: Internal controls for WB

I'm glad you started this post, as I have a similar question I'd like to throw out (sorry, no answer).

Please see my thread here:
[Only registered users see links. ]

Last edited by admin; 01-09-2008 at 03:43 PM.
Reply With Quote
  #5  
Old 01-09-2008, 03:45 PM
Volunteer
Points: 2,675, Level: 33 Points: 2,675, Level: 33 Points: 2,675, Level: 33
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Feb 2006
Posts: 48
Thanks: 0
Thanked 6 Times in 4 Posts
Default Re: Internal controls for WB

Hello there,

I have has similar issues with western blots.

The best thing to do is try two internal controls such as alpha tubulin and beta actin.

Then you can use one as a loading control and the other as an internal control


Always check to see if any of the internal controls are affecting in different tissues or treatments.
Reply With Quote
Reply

Tags
controls , internal


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
test if there is a difference between cases and controls SSN BioStatistics Forum 0 12-21-2009 02:38 AM
Internal Microarray Controls? albinpaul Microarrays Forum 0 07-23-2008 12:12 PM
problem with a negative controls Anna Protocols and Methods Forum 4 08-01-2004 06:51 AM
New Saccharomyces Sequences 02/25/04 SGD Sequences Yeast Forum 0 02-27-2004 04:23 AM
Internal calcium: How might a cell detect fluctuations of internal ca2+??? tomte Cell Biology and Cell Culture 0 07-29-2003 03:48 PM


All times are GMT. The time now is 08:09 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.13368 seconds with 16 queries