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| Western Blot Forum Discuss western blotting and immunoblot in the western blot forum. |
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| I am new to this forum, and I found it is very good site to get advice on specific issues. I'm working on a project to examine gene expressions in mouse tissues (liver, muscles etc) by Western Blots. I'm wondering which internal controls I should use? actin? tubulin? GAPDH? or something else? |
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| Hello there, I have has similar issues with western blots. The best thing to do is try two internal controls such as alpha tubulin and beta actin. Then you can use one as a loading control and the other as an internal control ![]() Always check to see if any of the internal controls are affecting in different tissues or treatments. |
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| controls , internal |
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