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Western Blot Forum Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


Western Blot Densitometry

Western Blot Densitometry - Western Blot Forum

Western Blot Densitometry - Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


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  #1  
Old 08-10-2006, 11:23 PM
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Arrow Western Blot Densitometry



Please post your tips on densitmetry of western blot bands. How do you analyze your figures?

I am using spot densitometry with boxes of the same size subracting background boxes for each band.

thanks in advance
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Old 02-24-2007, 04:06 AM
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Default Re: Western Blot Densitometry

Hey Moleculardude,

Make sure when conducting densitometry that all the boxes for the bands are the same size.

Also all the boxes for the background analysis should be the same size as well.

Try and fix the boxes in similar areas as moving them around will change the densitometry analysis.

cheers!
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Old 02-26-2007, 07:28 PM
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Default Re: Western Blot Densitometry

Try to use TotalLab program. It is easy steps to calculate your bands
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