Can we use dot blot inplace of Western blot?

[peterish]

You know dot blot is much easy to do than Western blotting, and in few occasions that we will lose the signal. Should we use dot blot to replace Western blotting?
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On the other hand, I wonder if dot blot can be used for samples eluted from immunoprecipitation. Your help and opinion is respected.

[danfive]

Yes, dot blot is excellent. Especially if you can document that the Antibody detects your target protein in the Western, and the detection is specific----no nonspecific bands. So if you have a great Western blot set up for your target protein, go ahead and begin doing dot-blots.

[peterish]

I got it. Thank you for your tips.