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SDS-PAGE Voltage not Ramping Up

SDS-PAGE Voltage not Ramping Up - Western Blot Forum

SDS-PAGE Voltage not Ramping Up - Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


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Old 10-27-2008, 07:42 PM
Pipette Filler
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Default SDS-PAGE Voltage not Ramping Up



Trying to run an SDS-PAGE for western. Problem is, I set constant voltage to 150 and notice that it switches to constant amps, and the actual voltage will never get past about 60. I've tried a different tank/lid/cassette type and it seems to work about half the time (the other lid/set will almost never work). I seem to also be getting this problem with another power supply as well. I've tried making up the running-buffer (glycine/SDS) three times already.

What could cause this problem? (Buffer, Lid, Casette, power supply???)
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Old 10-31-2008, 06:05 AM
Pipette Filler
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Default Re: SDS-PAGE Voltage not Ramping Up

what is the brand of the power supply you use? Bio-Rad?

There is a button, written " V | A " this allows you to let the power supply run on constant voltage or constant current.
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