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Collagen type 1 expression, help needed

Collagen type 1 expression, help needed - Western Blot Forum

Collagen type 1 expression, help needed - Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


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Old 12-13-2007, 05:11 AM
Pipette Filler
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Default Collagen type 1 expression, help needed



Hello everybody,
I am doing Western blotting to detect Collagen type 1 expression after stimulation with growth factor. And I will use mouse lung fibroblast.
I used many antibodies for collagen type 1, but still can not get the bands. Does someone have experience with collagen type 1? please advice me
1. which antibody (from what maker?) did you use?

Last edited by Handa; 12-13-2007 at 05:18 AM.
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Old 06-24-2010, 11:38 PM
Pipette Filler
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Default Re: Collagen type 1 expression, help needed

i've been using a rabbit collagen antibody from Rockland with great success for detecting type I. we use cell lysate from human fibroblasts. rockland also sells the secondary (anti-rabbit HRP) which works very well in my experience.

hope this helps
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