| |||||||
| Register | Search | Today's Posts | Mark Forums Read |
| Western Blot Forum Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures. |
 |
| | LinkBack | Thread Tools | Display Modes |
|
#1
11-26-2007, 07:42 PM
| |||||||||||
| |||||||||||
 Western Blotting reagents Simply want to know that all reagents should be made in autoclaved distill water or simply distilled water?  |
|
#2
12-02-2007, 08:06 AM
| |||||||||||
| |||||||||||
 Re: Western Blotting reagents Simply distilled water will do. You do not do the reaction under sterile conditions so there is no point in autocalving the water. Check the pH of water occasionaly so you are sure the pH is not diverging from what it should be. good luck |
|
| Tags |
| blotting , reagents , western |
| Thread Tools | |
| Display Modes | |
|
|
Similar Threads | ||||
| Thread | Thread Starter | Forum | Replies | Last Post |
| How to optimise Western blotting for eNOS detection? | plummy | Molecular Biology Techniques | 4 | 04-17-2013 02:11 PM |
| Difference between Western Blotting and Immunoprecipitation? | tonyz06 | Protein Science | 5 | 04-23-2010 07:33 PM |
| Advice needed for Western blotting | Peter Ellis | Protocols and Methods Forum | 1 | 06-17-2008 09:41 PM |
| Novel(?) Western Blotting question | fitbrit | Western Blot Forum | 7 | 09-18-2007 08:09 PM |
| western blotting help (leelavati shetty) | Tsalavos Sotiris | Protein Forum | 0 | 01-08-2007 12:50 PM |
Linear Mode
