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Western Blot Forum Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


Western Blotting reagents

Western Blotting reagents - Western Blot Forum

Western Blotting reagents - Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


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Old 11-26-2007, 08:42 PM
Pipette Filler
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Question Western Blotting reagents



Simply want to know that all reagents should be made in autoclaved distill water or simply distilled water?
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Old 12-02-2007, 09:06 AM
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Default Re: Western Blotting reagents

Simply distilled water will do. You do not do the reaction under sterile conditions so there is no point in autocalving the water. Check the pH of water occasionaly so you are sure the pH is not diverging from what it should be.

good luck
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