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IP washing step

Western Blot Forum Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.

IP washing step

IP washing step - Western Blot Forum

IP washing step - Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.

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#11

12-11-2007, 10:32 PM

danfive

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Re: IP washing step

Quote:

Originally Posted by bobbylee

Have never had a problem. Get a nice band with no background/non-specific binding when running a western.

Well yeah, but this thread refers to IP not WB.

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« Convex shape of sample bands on SDS PAGE gel | Non-specific antibody. »

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IP washing step

IP washing step - Western Blot Forum

IP washing step - Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.