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| Western Blot Forum Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures. |
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#1
11-10-2007, 03:25 AM
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 Western Blot Membrane Incubation with NP-40? Email question from a user: When performing a ligand blot analysis, under non-reducing conditions, after western blotting, the nitocellulose membrane is incubated in 3% NP-40 for half hour, followed by blocking in BSA in 0.1% Tween PBS. I have come across many papers where NP40 is used. Can you explain to me why this is done? Thank you. Kind regards. (posted by admin)   |
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#2
11-10-2007, 08:33 PM
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 Re: Western Blot Membrane Incubation with NP-40? Because both NP-40 (Igepal) and Tween-20 are both weak nonionic surfactants used in wash solutions for Westerns, ISH, Dot-blots, ELISA. Along with Triton X; another nonionic surfactant, they dissolve easily in TBS, or PBS solutions. |
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#3
11-10-2007, 08:56 PM
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| Re: Western Blot Membrane Incubation with NP-40? For more background you may want to read "A Guide to the Properties and Uses of Detergents in Biology and Biochemistry" by Srirama M. Bhari --> [Only registered users see links. ] |
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| blot , incubation , membrane , np40 , western |
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