quantify disturbed gel

[ofir]

Hi
I working on a very sticky protein (A-beta), which result in a very disturbed gel. The file that I get in the end is very dirty so it's extremely difficult to quantify the bands. Currently I am using software that’s called TINA I have also tried image J none of which are working to well, do anyone have any suggestions? Someone told me about new software that he is using, EZQuant-Gel did anyone try it?
Thanks

[DNA_science]

Hello. There are many programs that will delete (subtract) the background next to the band (using a box).

All you have to do is use a gel densitometry measurement software for analysis. Just use the same box size for your bands, and a smaller box (same size for all) next the bands (but outside them) for your background.

NO problem