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#11
02-05-2010, 07:08 AM
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 Re: Lysis for WB with Ripa Buffer - too viscous, no pellet - urgent help needed, plea In my lab we use a NP40, and then we add laemelli with DDT directly to the wells before we scrape. I am not getting consistent protein between my wells in my 96 well plates. I would like to use an alternative method of lysis so that I can get nuclear proteins, but I can quantify my proteins before using for a western. Can I use Ripa for this and then add my laemelli just before loading?  |
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#12
02-01-2013, 04:35 PM
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| Re: Lysis for WB with Ripa Buffer - too viscous, no pellet - urgent help needed, plea Thanks Laura, Just a quick question, do you recommend any voltage for sonication? |
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#13
02-01-2013, 05:03 PM
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| Re: Lysis for WB with Ripa Buffer - too viscous, no pellet - urgent help needed, plea Thanks Laura, Just a quick question, do you recommend any voltage for sonication? |
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| Tags |
| buffer , lysis , needed , pellet , ripa , urgent , viscous |
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