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Storage of PVDF membranes after western

Storage of PVDF membranes after western - Western Blot Forum

Storage of PVDF membranes after western - Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


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  #1  
Old 10-03-2007, 03:48 AM
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Red face Storage of PVDF membranes after western



Hi there,

does anybody have a tip how to store the PVDF membranes after the first development and before the next stripping?

Thanks!
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Old 10-03-2007, 04:33 PM
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Default Re: Storage of PVDF membranes after western

You can wash them and dry them, or store them in wash or blocking buffer.
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Old 10-04-2007, 02:24 AM
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Smile Re: Storage of PVDF membranes after western

Thank you for your answer. If we dry them, will they work next time? Which volume should I keep it in the buffer- just enough to cover it?
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Old 10-04-2007, 02:39 PM
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Default Re: Storage of PVDF membranes after western

Quote:
Originally Posted by cintiadp View Post
Thank you for your answer. If we dry them, will they work next time? Which volume should I keep it in the buffer- just enough to cover it?
Yeah just enough to cover it. Yes the dried membranes will work again but you will have to activate the membrane by immersing it in methanol first. Some research says that the methanol step harms the protein and later western results---but this is totally dependent on your protein.
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Old 10-07-2007, 03:19 AM
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Default Re: Storage of PVDF membranes after western

How long have you kept your membranes for later use? And how many times have you stripped it?
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Old 10-08-2007, 02:33 PM
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Default Re: Storage of PVDF membranes after western

Quote:
Originally Posted by cintiadp View Post
How long have you kept your membranes for later use? And how many times have you stripped it?
I've stored membranes for two weeks wet and they performed fine; dry they should be stable for 6-12 months (so I heard). I've stripped as many as two times (basic pH). Never tried for more than two weeks or stripping more than twice, so they may last longer and endure more stripping, I don't know.
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Old 10-10-2007, 12:26 PM
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Default Re: Storage of PVDF membranes after western

I called the company from were I buy my membrane and asked them the very question you asked. I have a PVDF transfer membrane and they told me to NOT dry it out. If I want to keep it I should put it in TBS in the fridge.
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Old 10-10-2007, 04:05 PM
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Default Re: Storage of PVDF membranes after western

Quote:
Originally Posted by Towe View Post
I called the company from were I buy my membrane and asked them the very question you asked. I have a PVDF transfer membrane and they told me to NOT dry it out. If I want to keep it I should put it in TBS in the fridge.
That is good advice, keeping the membranes wet is my preferred method as well.
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