How to Store Western Blot Membranes Later Use

[pipette_man]

Hi everyone!
Does anyone know how one can store western blot membranes for later use?

I want to run some gels and transfer them to membranes, before the weekend to blot on Monday.

Thanks!

[danfive]

You can air dry after the transfer and store in safe place protein side up. Don't touch membrane with bare hands. You can then "activate" the membrane by dipping it in methanol for a few seconds then do a wash step with wash buffer, block, etc.

If you prefer not to air dry, you can just keep you membrane wet in wash buffer or blocking buffer and store at 4C, until ready.