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Western Blot Forum Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


Membrane Stripping and Number of Times you can Blot

Membrane Stripping and Number of Times you can Blot - Western Blot Forum

Membrane Stripping and Number of Times you can Blot - Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


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Old 07-15-2006, 06:47 PM
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How many times can I strip a membrane before I should stop using it? I have tried up to 3 or 4 times and it still works ok. any ideas for this?

thank u
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Old 07-19-2006, 07:49 PM
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Default Re: Membrane Stripping and Number of Times you can Blot

I think it is ok to use it as many times as you can. You should stop using it when you have no signal any more. My record was 3 times, but just because of no need to blot it further. So, you are the recordsman!

Alexei.
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Old 07-19-2006, 09:52 PM
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Default Re: Membrane Stripping and Number of Times you can Blot

Hi! and Thank you for this! I think I am lucky as I blot for total protein...

phosphorylation antibody wouldn't work even with 1 stripping... (best to do that first! )
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