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Bcl-2 is too weak

Bcl-2 is too weak - Western Blot Forum

Bcl-2 is too weak - Discuss western blotting and immunoblot in the western blot forum.


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Old 07-21-2007, 08:01 AM
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Question Bcl-2 is too weak



Why my Bcl-2 strip is always too weak even cannot seen?
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Old 10-29-2007, 07:39 AM
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Default Re: Bcl-2 is too weak

Hello there Bill,

it may be you do not have enough protein loaded. Try lysing each sample/well/dish in 100ul of SDS-PAGE loading buffer. Load this whole amount onto your gel lanes.

cheers
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