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Invitrogen and stripping

Invitrogen and stripping - Western Blot Forum

Invitrogen and stripping - Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


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Old 05-09-2007, 06:07 PM
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Default Invitrogen and stripping



Why is it that you can not strip Invitrogen NC blots? Which manufacturer offers the best NC to yield good stripping characteristics?
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Old 05-28-2007, 04:07 PM
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Default Re: Invitrogen and stripping

Hi...did you try to strip membrane? Maybe it works! You can try different stripping buffers...
I use Hybond P+ membrane, Amersham, and it works good for 2-3 strippings. Why don't you try to use pvdf membrane? Sometimes I use Hybond N+ Amersham...
Good luck
Mara
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Old 05-29-2007, 08:00 PM
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Default Re: Invitrogen and stripping

Thanks Mara, I did try to strip the Invitrogen NC blots and did not get any results. I then looked at Invitrogen literature and saw a note that did not recommend stripping of their NC membranes. I now use Biorad and now get good stripping of their membrane.
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