| |||||||
| Register | Search | Today's Posts | Mark Forums Read |
| Western Blot Forum Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures. |
 |
| | LinkBack | Thread Tools | Display Modes |
|
#1
06-26-2006, 07:54 PM
| ||||||||||||
| ||||||||||||
 Blocking Membranes for Western Blotting what is the best blocking agent for membrane western blotting? is Blotto (milk) the best or casein and what about BSA??!  do i need to try them all first?   |
|
#3
08-10-2006, 07:58 PM
| |||||||||||
| |||||||||||
 Re: Blocking Membranes for Western Blotting In my experience 5% milk in 1x TBS works the best. It is important to dissolve milk completely, otherwise milk clumps will cause nasty speckles on the film. If blocking is performed overnigt at 4C it is recommended to add some sodium azide. |
|
| Tags |
| blocking , blotting , membranes , western |
| Thread Tools | |
| Display Modes | |
|
|
Similar Threads | ||||
| Thread | Thread Starter | Forum | Replies | Last Post |
| Recommended Western Blot Membrane Supplier | labratty | Western Blot Forum | 4 | 01-24-2009 12:40 PM |
| questions about functional blocking antibodies | wangleicau | Cell Biology and Cell Culture | 1 | 07-03-2008 02:58 AM |
| questions about functional blocking antibodies | wangleicau | Cell Biology and Cell Culture | 0 | 05-16-2008 07:24 PM |
| 1 questions about functional blocking antibodies | wangleicau | Cell Biology and Cell Culture | 0 | 05-16-2008 07:23 PM |
| Blocking of epoxy-coated slides | Sanjay Vijendran | Protocols and Methods Forum | 2 | 10-05-2005 12:41 PM |
Linear Mode
