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Western Blot Forum

Western Blot Forum Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.

Western Blot Forum

Western Blot Forum - Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.

Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.

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buffer, lysis, needed, pellet, ripa, urgent, viscous

Sticky: Lysis for WB with Ripa Buffer - too viscous, no pellet - urgent help needed, please (

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02-01-2013 05:03 PM
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Sticky: Western Blot Optimization (

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04-29-2011 09:15 AM
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blotting, semi-dry, semidry, western blot

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03-25-2011 12:10 AM
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amount, blot, calculate, gel, load, protein, western

Sticky: How to calculate amount of protein to load into Western Blot gel

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blotting, phosphorylated, proteins, western

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02-20-2011 12:38 PM
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blot, control, protein, secreted, western

Sticky: Western blot secreted protein control

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02-20-2011 12:33 PM
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bands, disappears, phospho, problem, quickly, signal, western

Sticky: Problem: phospho signal quickly disappears

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11,805

Western for IgA

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Mouse Liver Actin Blot

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03-06-2013 10:13 AM
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163

diabetic, expression, nfkb, normal, vegf, wound

Expression of NFKB and VEGF in diabetic wound Vs Normal wound

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157

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03-05-2013 05:17 PM
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140

bands, blot, inconsistent, marker, slant, western

Slant, inconsistent marker bands in Western blot

gsb1989

03-05-2013 03:27 PM
by admin

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blot, signal, stripping, stronger, western

Stronger signal after stripping my western blot

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02-15-2013 10:03 AM
by research_ER

209

5kd, blot, difference, proteing, separating, western

Separating proteing with difference of 5Kd on western blot

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02-11-2013 10:52 AM
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215

Mysteriously disappeared bands

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02-02-2013 11:03 AM
by Immunola

first Antibody

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01-20-2013 02:43 AM
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229

western blot troubleshooting

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01-15-2013 12:54 PM
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Transfer 2kD peptide

wpstep

01-09-2013 06:36 PM
by DannyB.

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aureus, bacteria, blot, bradford assay, coli, e. coli, s. aureus, western, western blot

E. coli and S. aureus Western Blot

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GAPDH bad signal

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11-30-2012 10:58 AM
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205

assay, concentration, protein, stability, tetracycline

Tetracycline concentration for protein stability assay?

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244

Western blot for E.coli

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11-15-2012 06:53 PM
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Strong band but wrong molec. weight?

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11-08-2012 05:26 PM
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Band showing up at wrong molec. weight?

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11-08-2012 05:20 PM
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10-17-2012 11:01 AM
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problems, pvdf membrane, transfer, transfer problem, western transfer

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Western Blot Forum

Western Blot Forum - Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.

Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.