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#1
12-18-2012, 09:34 AM
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 RK13 transfection Hi guys, I have a strange problem with my RK13 cells. I wan't to generate a viral BAC, so I'm trying to transfect RK13 cells with viral DNA. I'm using PEI for transfection, but after 18 h incubation the cells look really crappy. Round and dark and sort of dead. But when I use a control or my transfer plasmid only there is no such effect. So I was wondering if anyone of you has encountered the same problem or has an idea of what that might be. Thank you very much in advance.  |
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