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Transfection Forum Transfection Forum. Cell transfection discussion including stable cells transfection, transient transfection. Troubleshooting and posting of discussions on lipofection, and reagents: Fugene, lipofectamine, and others.


RK13 transfection

RK13 transfection - Transfection Forum

RK13 transfection - Transfection Forum. Cell transfection discussion including stable cells transfection, transient transfection. Troubleshooting and posting of discussions on lipofection, and reagents: Fugene, lipofectamine, and others.


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Old 12-18-2012, 10:34 AM
Pipette Filler
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Default RK13 transfection



Hi guys,

I have a strange problem with my RK13 cells. I wan't to generate a viral BAC, so I'm trying to transfect RK13 cells with viral DNA. I'm using PEI for transfection, but after 18 h incubation the cells look really crappy. Round and dark and sort of dead. But when I use a control or my transfer plasmid only there is no such effect. So I was wondering if anyone of you has encountered the same problem or has an idea of what that might be.

Thank you very much in advance.
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