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Transfection Forum Transfection Forum. Cell transfection discussion including stable cells transfection, transient transfection. Troubleshooting and posting of discussions on lipofection, and reagents: Fugene, lipofectamine, and others.


Transfection MDBKs

Transfection MDBKs - Transfection Forum

Transfection MDBKs - Transfection Forum. Cell transfection discussion including stable cells transfection, transient transfection. Troubleshooting and posting of discussions on lipofection, and reagents: Fugene, lipofectamine, and others.


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Old 10-23-2012, 12:59 PM
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Default Transfection MDBKs



Hey fellow researchers,

I am desperatly trying to transfect MDBK cells. I've tried several chemical compounds like PEI and Lipofectamin as well as Calcium Phosphate. None of those methods yielded any positive results. Than I tried electroporation and although I get a good efficiency when using pEGFP, I get nothing when I try to transfect my plasmid (it is pretty big, aroung 13 kbp and contains a GFP cassette). I found a couple of publications that describe successful transfection with both Calcium Phosphate and electroporation but I haven't been able to reproduce those results. Does anyone have experience with MDBK cells and transfection and maybe has a hint for me?

Thank you very much in advance.

Cheers,

Biotob
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