| |||||||
| Register | Search | Today's Posts | Mark Forums Read |
| Transfection Forum Transfection Forum. Cell transfection discussion including stable cells transfection, transient transfection. Troubleshooting and posting of discussions on lipofection, and reagents: Fugene, lipofectamine, and others. |
 |
| | LinkBack | Thread Tools | Display Modes |
|
#1
10-23-2012, 12:59 PM
| |||||||||||
| |||||||||||
 Transfection MDBKs Hey fellow researchers, I am desperatly trying to transfect MDBK cells. I've tried several chemical compounds like PEI and Lipofectamin as well as Calcium Phosphate. None of those methods yielded any positive results. Than I tried electroporation and although I get a good efficiency when using pEGFP, I get nothing when I try to transfect my plasmid (it is pretty big, aroung 13 kbp and contains a GFP cassette). I found a couple of publications that describe successful transfection with both Calcium Phosphate and electroporation but I haven't been able to reproduce those results. Does anyone have experience with MDBK cells and transfection and maybe has a hint for me? Thank you very much in advance. Cheers, Biotob  |
|
| Tags |
| mdbks , transfection |
| Thread Tools | |
| Display Modes | |
|
|
Similar Threads | ||||
| Thread | Thread Starter | Forum | Replies | Last Post |
| siRNA transfection in HL-60 cells | oldach | RNAi and SiRNA Forum | 0 | 11-11-2011 02:47 AM |
| development of siRNA Transfection reagent!!!!!!!! | Behiye | RNAi and SiRNA Forum | 0 | 08-09-2011 01:52 PM |
| Any Transfection kit for HepG2 cells? | drkashyap | Transfection Forum | 5 | 02-25-2009 10:10 PM |
| Transfection myoblast | laure d'astarac | Protocols and Methods Forum | 0 | 09-24-2008 01:55 PM |
| Cell Adhesion Problem After Transfection | gandalfthegrey | Cell Biology and Cell Culture | 1 | 04-09-2008 02:40 PM |
Linear Mode
