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Transfection Forum Transfection Forum. Cell transfection discussion including stable cells transfection, transient transfection. Troubleshooting and posting of discussions on lipofection, and reagents: Fugene, lipofectamine, and others.


infection after 96 hours of GP293 cells transfections?

infection after 96 hours of GP293 cells transfections? - Transfection Forum

infection after 96 hours of GP293 cells transfections? - Transfection Forum. Cell transfection discussion including stable cells transfection, transient transfection. Troubleshooting and posting of discussions on lipofection, and reagents: Fugene, lipofectamine, and others.


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Old 02-23-2012, 05:08 PM
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Default infection after 96 hours of GP293 cells transfections?



Hello I have an urgent question and I would be very grateful if somebody can reply me.

I have made several transfections in GP293 cells and I wanted to infect the target cells after 48 hours of tranfections. This is the optimal time transfection and I know that you can also infect after 72 hours of transfection. But my problem is that today I have made the seed of target cells and I believe that tomorrow they will not be in a good confluence. It's probably that I would wait one more day to get the minium confluence of my target cells.

So, my question is: Can I do my infection after 96 hours of GP293 cells transfections?

Thanks a lot.
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