Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Cell Biology and Cell Culture > Transfection Forum
Register Search Today's Posts Mark Forums Read

Transfection Forum Transfection Forum. Cell transfection discussion including stable cells transfection, transient transfection. Troubleshooting and posting of discussions on lipofection, and reagents: Fugene, lipofectamine, and others.


Cells dying after limit dilution?

Cells dying after limit dilution? - Transfection Forum

Cells dying after limit dilution? - Transfection Forum. Cell transfection discussion including stable cells transfection, transient transfection. Troubleshooting and posting of discussions on lipofection, and reagents: Fugene, lipofectamine, and others.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 01-10-2012, 02:47 PM
Pipette Filler
Points: 52, Level: 1 Points: 52, Level: 1 Points: 52, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jan 2012
Location: Roanoke, VA
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Question Cells dying after limit dilution?



Hi all-

I'm trying to generate a stable cell line using nucleofected C3H cells under G418 selection. Everything goes great until I do a limit dilution to select for single cells- at this point, the cells are all dying. I'm guessing it's due to the single cell not being able to properly condition the media. Any suggestions on how to fix this? I've thought of using a different media- maybe Ham's cloning, but I have no experience in this so I thought I'd ask.

Thanks for any help!

-Anne
Reply With Quote
Reply

Tags
cells , dilution , dying , limit


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
mouse hepatocytes isolation amicia Cell Biology and Cell Culture 39 05-11-2012 11:52 AM
Characteristics of cancerous cells aftabac Cell Biology and Cell Culture 2 08-26-2011 11:40 PM
Human Cytome Project - Update 24 Jan. 2005 Peter Van Osta Cell Biology and Cell Culture 1 08-01-2010 02:18 PM
Human Cytome Project - Update 6 Jan. 2005 Peter Van Osta Cell Biology and Cell Culture 0 01-06-2005 11:18 AM


All times are GMT. The time now is 10:34 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.14374 seconds with 16 queries