Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Cell Biology and Cell Culture > Transfection Forum
Register Search Today's Posts Mark Forums Read

Transfection Forum Transfection Forum. Cell transfection discussion including stable cells transfection, transient transfection. Troubleshooting and posting of discussions on lipofection, and reagents: Fugene, lipofectamine, and others.


NGf-differentiated Pc12 and siRNA transfection problem

NGf-differentiated Pc12 and siRNA transfection problem - Transfection Forum

NGf-differentiated Pc12 and siRNA transfection problem - Transfection Forum. Cell transfection discussion including stable cells transfection, transient transfection. Troubleshooting and posting of discussions on lipofection, and reagents: Fugene, lipofectamine, and others.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 11-21-2011, 09:23 AM
Pipette Filler
Points: 52, Level: 1 Points: 52, Level: 1 Points: 52, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Nov 2011
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Default NGf-differentiated Pc12 and siRNA transfection problem



This is my problem
In my these, I will Differentiate PC12 cell lines with NGF ( within 7 days) to Neuronal like cell, and then tranfect Neuronal like cells with two different siRNAs. because tranfection rates of these differentiated cells is to some extent low and difficult, is any body please let me know about having any data, report, information, paper or method concerning suitable siRNA transfection reagents for this type of neuronal like cell derived by Pc12 differentiation and any other your kindly suggestion for solving my problem.

Sincerely
Reply With Quote
Reply

Tags
ngfdifferentiated , pc12 , problem , sirna , transfection


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump


All times are GMT. The time now is 11:31 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11151 seconds with 15 queries