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cell culture for ELISA
I'm trying running a ELISA with cell culture supernatants, and my results are all over the place.
I'm pretty confident in my Elisa - its a commercial precoated kit and my standard curve was fine - so that only leaves errors in the culture technique.
What could I be doing wrong that would lead to inconsistant results in the ELISA?
I'm using 16HBE cells (adherent), and testing PGE2 levels after stimulation and inhibition. Supernatants are collected after 4 and 24 hours
If you would like more specific details, let me know!
Re: cell culture for ELISA
Either way 1st thing will be to do a dilution series. Analyze the results: Random signal up/down = interference, hook effect = protein associating together at high density, no signal = inhibition (could be due to pH)
|cell , culture , elisa|
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