I'm trying running a ELISA with cell culture supernatants, and my results are all over the place.
I'm pretty confident in my Elisa - its a commercial precoated kit and my standard curve was fine - so that only leaves errors in the culture technique.
What could I be doing wrong that would lead to inconsistant results in the ELISA?
I'm using 16HBE cells (adherent), and testing PGE2 levels after stimulation and inhibition. Supernatants are collected after 4 and 24 hours
If you would like more specific details, let me know!