Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Cell Biology and Cell Culture > Transfection Forum
Register Search Today's Posts Mark Forums Read

Transfection Forum Transfection Forum. Cell transfection discussion including stable cells transfection, transient transfection. Troubleshooting and posting of discussions on lipofection, and reagents: Fugene, lipofectamine, and others.


cell culture for ELISA

cell culture for ELISA - Transfection Forum

cell culture for ELISA - Transfection Forum. Cell transfection discussion including stable cells transfection, transient transfection. Troubleshooting and posting of discussions on lipofection, and reagents: Fugene, lipofectamine, and others.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 10-17-2011, 04:10 AM
Pipette Filler
Points: 24, Level: 1 Points: 24, Level: 1 Points: 24, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Oct 2011
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Default cell culture for ELISA



Hi all!

I'm trying running a ELISA with cell culture supernatants, and my results are all over the place.
I'm pretty confident in my Elisa - its a commercial precoated kit and my standard curve was fine - so that only leaves errors in the culture technique.
What could I be doing wrong that would lead to inconsistant results in the ELISA?

I'm using 16HBE cells (adherent), and testing PGE2 levels after stimulation and inhibition. Supernatants are collected after 4 and 24 hours
If you would like more specific details, let me know!
Reply With Quote
  #2  
Old 11-10-2011, 07:41 PM
danfive's Avatar
Nobel Laureate
Points: 8,612, Level: 64 Points: 8,612, Level: 64 Points: 8,612, Level: 64
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jul 2007
Location: Houston TX
Posts: 969
Thanks: 12
Thanked 184 Times in 154 Posts
Post Re: cell culture for ELISA

Quote:
Originally Posted by mywillowtree View Post
Hi all!

I'm trying running a ELISA with cell culture supernatants, and my results are all over the place.
I'm pretty confident in my Elisa - its a commercial precoated kit and my standard curve was fine - so that only leaves errors in the culture technique.
What could I be doing wrong that would lead to inconsistant results in the ELISA?

I'm using 16HBE cells (adherent), and testing PGE2 levels after stimulation and inhibition. Supernatants are collected after 4 and 24 hours
If you would like more specific details, let me know!
Are you talking about conditioned media or supernatant of cell culture lysate?
Either way 1st thing will be to do a dilution series. Analyze the results: Random signal up/down = interference, hook effect = protein associating together at high density, no signal = inhibition (could be due to pH)
Reply With Quote
  #3  
Old 01-11-2013, 07:38 PM
Graduate Student
Points: 247, Level: 4 Points: 247, Level: 4 Points: 247, Level: 4
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jan 2013
Posts: 100
Thanks: 0
Thanked 0 Times in 0 Posts
Default バーバリー ポロシャツ

[

[

[
Reply With Quote
  #4  
Old 01-11-2013, 07:56 PM
Graduate Student
Points: 247, Level: 4 Points: 247, Level: 4 Points: 247, Level: 4
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jan 2013
Posts: 100
Thanks: 0
Thanked 0 Times in 0 Posts
Default バーバリー アウト レット

[

[

[
Reply With Quote
Reply

Tags
cell , culture , elisa


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Human Cytome Project - Update 24 Jan. 2005 Peter Van Osta Cell Biology and Cell Culture 1 08-01-2010 02:18 PM
Human Cytome Project - an idea - Update 19 April 2005 Peter Van Osta Cell Biology and Cell Culture 1 06-01-2009 02:17 PM
A Human Cytome Project - an idea - Update 14 March 2005 Peter Van Osta Cell Biology and Cell Culture 0 03-14-2005 02:27 PM
Human Cytome Project - Update 6 Jan. 2005 Peter Van Osta Cell Biology and Cell Culture 0 01-06-2005 11:18 AM
Tissue Engineering & Stem Cell Research 2005 Andrew Oliver Protocols and Methods Forum 0 12-02-2004 11:38 PM


All times are GMT. The time now is 03:27 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.18540 seconds with 16 queries