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HPAF II transient transfection
I am trying to transiently transfect HPAF II cells (human pancreatic), but efficiency is very low (5%). Any suggestions or protocols for better transfection rates around? What efficiency can I expect?
Re: HPAF II transient transfection
I´m also searching for more efficience in HPAF II cells. which reagent are you using. they don´t like all!! how much cells you´ve plate before??? did you check also reverse transfection. i have more efficience by transfection with si RNA than DNA...
|hpaf , transfection , transient|
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