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Transfection Forum Transfection Forum. Cell transfection discussion including stable cells transfection, transient transfection. Troubleshooting and posting of discussions on lipofection, and reagents: Fugene, lipofectamine, and others.


HPAF II transient transfection

HPAF II transient transfection - Transfection Forum

HPAF II transient transfection - Transfection Forum. Cell transfection discussion including stable cells transfection, transient transfection. Troubleshooting and posting of discussions on lipofection, and reagents: Fugene, lipofectamine, and others.


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Old 06-28-2011, 12:27 PM
Pipette Filler
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Default HPAF II transient transfection



I am trying to transiently transfect HPAF II cells (human pancreatic), but efficiency is very low (5%). Any suggestions or protocols for better transfection rates around? What efficiency can I expect?
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Old 07-20-2011, 08:29 AM
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Default Re: HPAF II transient transfection

Quote:
Originally Posted by makirch View Post
I am trying to transiently transfect HPAF II cells (human pancreatic), but efficiency is very low (5%). Any suggestions or protocols for better transfection rates around? What efficiency can I expect?

Im also searching for more efficience in HPAF II cells. which reagent are you using. they dont like all!! how much cells youve plate before??? did you check also reverse transfection. i have more efficience by transfection with si RNA than DNA...
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