| |||||||
| Register | Search | Today's Posts | Mark Forums Read |
| Transfection Forum Transfection Forum. Cell transfection discussion including stable cells transfection, transient transfection. Troubleshooting and posting of discussions on lipofection, and reagents: Fugene, lipofectamine, and others. |
 |
| | LinkBack | Thread Tools | Display Modes |
|
#1
06-28-2011, 12:27 PM
| |||||||||||
| |||||||||||
 HPAF II transient transfection I am trying to transiently transfect HPAF II cells (human pancreatic), but efficiency is very low (5%). Any suggestions or protocols for better transfection rates around? What efficiency can I expect?  |
|
#2
07-20-2011, 08:29 AM
| |||||||||||
| |||||||||||
| Re: HPAF II transient transfection Quote:
I´m also searching for more efficience in HPAF II cells. which reagent are you using. they don´t like all!! how much cells you´ve plate before??? did you check also reverse transfection. i have more efficience by transfection with si RNA than DNA... |
|
| Tags |
| hpaf , transfection , transient |
| Thread Tools | |
| Display Modes | |
|
|
Similar Threads | ||||
| Thread | Thread Starter | Forum | Replies | Last Post |
| Stable transfection in suspension cells | Kamalian, Laleh | Protocols and Methods Forum | 12 | 03-01-2012 11:47 AM |
| Cell dies after transient transfection with lipo 2000 on HEK293 | liyaxu | Transfection Forum | 1 | 05-17-2009 09:05 AM |
| Transient Transfection Problem | TLF | Transfection Forum | 5 | 02-25-2009 10:17 PM |
| Any Transfection kit for HepG2 cells? | drkashyap | Transfection Forum | 5 | 02-25-2009 10:10 PM |
| Transfection myoblast | laure d'astarac | Protocols and Methods Forum | 0 | 09-24-2008 01:55 PM |
Linear Mode
