| | Re: HMEC-1 stable transfection: blasticidin resistance?
No, I have not found a solution to the problem. I have, however, meanwhile contacted the group which first immortalized them and atthey told me that they didn't use blasticin-selection, so in principle the cell line should not be resistant.
The only advice I can give you is to start pretty high!
If you find a suitable concentration in your titration experiments, would you mind telling me (I would repeat the titration anyway)?
Last edited by kraefzke; 07-26-2011 at 09:09 AM.